Figure 9

Two-color RNA-FISH in female fibroblasts reveals independent escape from inactivity of two neighboring X-borne loci. Loci are color coded above panels. (a) Nuclei in which one gene (green) is expressed from both alleles and the second gene (red) is expressed from only one allele. (b) Nuclei in which one gene (green) is expressed from one allele and the second gene (red) is expressed from both alleles. (c) Nuclei in which both genes are expressed from both alleles. ND, no nuclei were observed in this category. Nuclei are counterstained with DAPI (blue).